Two methods for simultaneous estimation of dorzolamide HCl and timolol maleate in two component ocular insert form have been developed. The methods employ the application of simultaneous equation and the absorbance ratio (Q-analysis) methods. Both methods utilize simulated tear fluid (STF) pH, 7.4 as solvent. Dorzolamide HCl and timolol maleate shows maximum absorbance at wavelengths at 254nm and 294.8nm respectively. Linearity ranges for both dorzolamide HCl and timolol maleate is 5 – 40 µg/ml. The isoabsorptive point is 276 nm. The procedures were successfully applied for the simultaneous estimation of both drugs in laboratory prepared ocular inserts. Results of both methods were consistent.
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